Composite

Part:BBa_K180010:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-21)

Rock-paper-scissors - Rock primary plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 916
    Illegal PstI site found at 2760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2760
    Illegal AgeI site found at 3365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147


Design Notes

BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.


Source

See relevant biobrick component parts (BBa_K180011 and BBa_K180012).

References